機器共用支援部門(CBS)	先進機器研究推進室(LPAR)
沿革	組織図	Staff	大型機器の紹介【動画】

先進機器共用推進部(ARFS)

先進機器研究推進室(LPAR)

LPARでは、ARFSの設備を活用し、学内の研究グループとの共同研究を推進することにより、本学の基礎および臨床医学研究に貢献することを目的としています。例えば、がんを主とするヒト疾患の原因となる、細胞周期制御因子の機能異常に注目し共同研究を行っています。

The aim of LPAR is to contribute to promoting the basic and clinical research through utilization of instruments in ARFS and inter-departmental collaboration. One of our collaborative topics is the dysfunction of the cell cycle regulators, which could be a cause of several human diseases including cancers and chronic disorders.

Contact:
Chiharu Uchida,Ph.D. AssociateProfessor
cuchida(at)hama-med.ac.jp

先進機器研究推進室に関連する主な論文 (2012～)

Uchida C*, Niida H, Sakai S, Iijima K, Kitagawa K, Ohhata T, Shiotani B, Kitagawa M.: p130RB2 positively contributes to ATR activation in response to replication stress via the RPA32-ETAA1 axis. Biochim. Biophys. Acta Mol. Cell Res. 1870(6):119484, 2023. doi: 10.1016/j.bbamcr.2023.119484. doi: 10.1111/pcmr.13102.
Goda M., Miyagi A., Kitamoto T., Kondo M. and Hashimoto H.: Uric acid is a major chemical constituent for the whitish coloration in the medaka leucophores. Pigment Cell Melanoma Res. 36(5): 416-422, 2023.
Ota K, Sakai S*, Ohhata T, Suzuki T, Uchida C, Niida H, Kitagawa M*.: APOBEC3B expression is promoted by lincNMR collaborating with TGF-β-Smad pathway. Carcinogenesis. 44(1):1-14, 2023. doi: 10.1093/carcin/bgac086
Ohhata T*, Suzuki M, Sakai S, Ota K, Yokota H,Uchida C, Niida H, Kitagawa M.: CCIVR facilitates comprehensive identification of cis-natural antisense transcripts with their structural characteristics and expression profiles. Sci Rep. 12(1):15525, 2022. doi: 10.1038/s41598-022-19782-5.
Koyauchi T, Niida H*, Motegi A, Sakai S,Uchida C, Ohhata T, Iijima K, Yokoyama A, Suda T, Kitagawa M.: Chromatin-remodeling factor BAZ1A/ACF1 targets UV damage sites in an MLL1-dependent manner to facilitate nucleotide excision repair. Biochim. Biophys. Acta Mol. Cell Res. 1869(11):119332, 2022. doi: 10.1016/j.bbamcr.
Tamura Y, Ohhata T*, Niida H, Sakai S,Uchida C, Masumoto K, Katou F, Wutz A, Kitagawa M*.: Homologous recombination is reduced in female embryonic stem cells by two active X chromosomes. EMBO Rep. 22(9):e52190, 2021, doi: 10.15252/embr.202052190.
Ohhata T*, Yamazawa K, Miura-Kamio A, Takahashi S, Sakai S, Tamura Y, Uchida C, Kitagawa K, Niida H, Hiratani I, Kobayashi H, Kimura H, Wutz A, and Kitagawa M: Dynamics of transcription-mediated conversion from euchromatin to facultative heterochromatin at the Xist promoter by Tsix. Cell Reports. 34, 108912, 2021, https://doi.org/10.1016/j.celrep.2021.108912
Kitagawa K*,Uchida C, Horiguchi R, Ohhata T, Sakai S, Niida H, Yasumoto S, Handa Y, Suzuki M, Hashimoto M, Tazawa T, Yokochi Y, Tsuji M, Kitagawa M*.: Substitution of Thr572 to Ala in mouse c-Myb attenuates progression of early erythroid differentiation. Sci Rep. 10(1):14381. 2020, doi: 10.1038/s41598-020-71267-5.
Nishimoto K, Niida H*,Uchida C, Ohhata T, Kitagawa K, Motegi A, Suda T, Kitagawa M.: HDAC3 Is Required for XPC Recruitment and Nucleotide Excision Repair of DNA Damage Induced by UV Irradiation. Mol. Cancer Res. 8(9):1367-1378, 2020. DOI: 10.1158/1541-7786.MCR-20-0214.
Sakai S, Ohhata T,Kitagawa K, Uchida C, Aoshima T, Niida H, Suzuki T, Inoue Y, Miyazawa K, Kitagawa M*: Long noncoding RNA ELIT-1 acts as a Smad3 cofactor to facilitate TGF-β/Smad signaling and promote epithelial-mesenchymal transition. Cancer Res. 79(11) 2821-2838, 2019, doi: 10.1158/0008-5472.CAN-18-3210.
Mikamo?M, Kitagawa K, Sakai S, Uchida C, Ohhata T, Nishimoto K, Niida H, Suzuki S, Nakayama KI, Inui N, Suda T, Kitagawa M*: Inhibiting Skp2 E3 ligase suppresses bleomycin-induced pulmonary fibrosis. Int J Mol Sci 19(2): E474-491, 2018. Doi:10.3390/ijms19020474
Niida H*, Matsunuma R, Horiguchi R, Uchida C, Nakazawa Y, Motegi A, Nishimoto K, Sakai S, Ohhata T, Kitagawa K, Moriwaki S, Nishitani H, Ui A, Ogi T, Kitagawa M.: Phosphorylated HBO1 at UV irradiated sites is essential for nucleotide excision repair. Nat Commun. Jul 18;8:16102. doi: 10.1038/ncomms16102, 2017.
Harada M, Sakai S, Ohhata T, Kitagawa K, Mikamo M, Nishimoto K, Uchida C, Niida H, Kotake Y, Sugimura H, Suda T, Kitagawa M.: Homeobox Transcription Factor NKX2-1 Promotes Cyclin D1 Transcription in Lung Adenocarcinomas. Mol Cancer Res. Oct;15(10):1388-1397, 2017.
Uchida C: Roles of pRB in the Regulation of Nucleosome and Chromatin Structures. Biomed Res. Int. Volume2016, Article ID5959721, 2016. Doi: 10.1155/2016/5959721
Kotake Y, Kitagawa K, Ohhata T, Sakai S, Uchida C, Niida H, Naemura M, Kitagawa M: Long Non-coding RNA, PANDA, Contributes to the Stabilization of p53 Tumor Suppressor Protein. Anticancer Res. 36(4):1605-1611, 2016.
Uchida C, Kitagawa M: RING-, HECT-, and RBR-type E3 Ubiquitin Ligases: Involvement in Human Cancer. Curr. Cancer Drug Targets 16(2):157-174, 2016.
Sakai S, Miyajima C, Uchida C, Itoh Y, Hayashi H, Inoue Y: Tribbles-Related Protein Family Members as Regulators or Substrates of the Ubiquitin-Proteasome System in Cancer Development. Curr. Cancer Drug Targets 16(2):147-156, 2016.
Matsunuma R, Niida H, Ohhata T, Kitagawa K, Sakai S, Uchida C, Shiotani B, Matsumoto M, Nakayama KI, Ogura H, Shiiya N, Kitagawa M: UV Damage-Induced Phosphorylation of HBO1 Triggers CRL4DDB2-Mediated Degradation To Regulate Cell Proliferation. Mol Cell Biol. 36(3): 394-406, 2015.
Nakajima T, Kitagawa K, Ohhata T, Sakai S, Uchida C, Shibata K, Minegishi N, Yumimoto K, Matsumoto A, Nakayama KI, Masumoto K, Katou F, Niida H, Kitagawa M: Regulation of GATA binding protein 2 levels via ubiquitin-dependent degradation by Fbw7: involvement of cyclin B-cyclin-dependent kinase 1-mediated phosphorylation of Thr176 in GATA binding protein 2.?J. Biol. Chem. 290:10370-10381, 2015.
Uchida C, Hattori T, Takahashi H, Yamamoto N, Kitagawa M, Taya Y: Interaction between RB protein and NuMA is required for proper alignment of spindle microtubules. Genes Cells. 19: 89-96, 2014.
Uchida C: The retinoblastoma protein: Functions beyond the G1-S regulator. Curr Drug Targets 13: 1622-1632, 2012.